Blocking Assay:Article Title: Local heterochromatin enrichment promotes telomere clustering and PML nuclear body assembly at telomeres.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine RNAiMAX Thermo Fisher Scientific Cat# 13778030 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000015 HMW Poly(I:C) InvivoGen Cat# tlrl-pic Protease QIAGEN Cat# 19157 Benzonase Nuclease EMD Millipore Cat# 70746-3 Formamide Millipore Sigma Cat# S4117 Blocking reagent Roche Cat# 11096176001 Phenol/Chloroform/Isoamyl alcohol (25:24:1) Acros Organics Cat# 32711500 Chloroform Fisher Chemical Cat# C574-1 100% Ethanol Decon Laboratories, Inc. Cat# 2701 Sodium Acetate VWR Cat# 0602-500G HinfI restriction enzyme New England Biolabs Cat# R0155L RsaI restriction enzyme New England Biolabs Cat# R0167 Φ29 Reaction Buffer New England Biolabs Cat# B0269S dNTP mix Qiagen Cat# 201913 Φ29 DNA Polymerase New England Biolabs Cat# M0629L CDP-Star Roche Cat# 11759051001 Pepsin Fisher Scientific Cat# A104-500 Paraformaldehyde, 16% w/v aq soln., methanol free Thermo Scientific Cat. No: 043368.9 RNase A Thermo Scientific Ref# EN0531 Protein A/G Magnetic Beads Thermo Scientific Cat# 88802 Protease inhibitor cocktail Roche Cat# 05892791001 ChIP protease inhibitor mix Diagenode Cat# C12010011 Nucleofection buffer MaxCyte Cat# EPB-1 Cas9 protein IDT Cat# 1081060 Neomycin Thermo Scientific J62671.03 Critical commercial assays BCA Protein Assay ThermoFischer 23227 DIG Oligonucleotide 3 ′ End Labeling Kit, 2ND generation Roche Cat# 03353575910 Qubit High Sensitivity dsDNA Kit Invitrogen Q32851 Click-iT EdU Cell Proliferation Kit for Imaging Thermo Fisher Scientific Cat# C10340 Qiagen DNA Purification Kit Qiagen Cat# 28106 DNA Clean and Concentrator 5-G Zymo Research D4013 Infusion Takara 638948 Zymoclean Gel DNA Recovery Kit Zymo Research D4001 Super Signal West Dura Genesee Scientific 20–301 Super Signal West Femto Genesee Scientific 20–302 Amersham Hybond P 0.2μm PVDF Cytiva 10600021 Bolt 4%–12% Bis-Tris Plus gels Invitrogen NW04120 Nucleofection Buffer MaxCyte EPB-1 Electroporation cuvette MaxCyte SOC-25x3 Experimental models: Cell lines
Magnetic Beads:Article Title: Local heterochromatin enrichment promotes telomere clustering and PML nuclear body assembly at telomeres.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine RNAiMAX Thermo Fisher Scientific Cat# 13778030 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000015 HMW Poly(I:C) InvivoGen Cat# tlrl-pic Protease QIAGEN Cat# 19157 Benzonase Nuclease EMD Millipore Cat# 70746-3 Formamide Millipore Sigma Cat# S4117 Blocking reagent Roche Cat# 11096176001 Phenol/Chloroform/Isoamyl alcohol (25:24:1) Acros Organics Cat# 32711500 Chloroform Fisher Chemical Cat# C574-1 100% Ethanol Decon Laboratories, Inc. Cat# 2701 Sodium Acetate VWR Cat# 0602-500G HinfI restriction enzyme New England Biolabs Cat# R0155L RsaI restriction enzyme New England Biolabs Cat# R0167 Φ29 Reaction Buffer New England Biolabs Cat# B0269S dNTP mix Qiagen Cat# 201913 Φ29 DNA Polymerase New England Biolabs Cat# M0629L CDP-Star Roche Cat# 11759051001 Pepsin Fisher Scientific Cat# A104-500 Paraformaldehyde, 16% w/v aq soln., methanol free Thermo Scientific Cat. No: 043368.9 RNase A Thermo Scientific Ref# EN0531 Protein A/G Magnetic Beads Thermo Scientific Cat# 88802 Protease inhibitor cocktail Roche Cat# 05892791001 ChIP protease inhibitor mix Diagenode Cat# C12010011 Nucleofection buffer MaxCyte Cat# EPB-1 Cas9 protein IDT Cat# 1081060 Neomycin Thermo Scientific J62671.03 Critical commercial assays BCA Protein Assay ThermoFischer 23227 DIG Oligonucleotide 3 ′ End Labeling Kit, 2ND generation Roche Cat# 03353575910 Qubit High Sensitivity dsDNA Kit Invitrogen Q32851 Click-iT EdU Cell Proliferation Kit for Imaging Thermo Fisher Scientific Cat# C10340 Qiagen DNA Purification Kit Qiagen Cat# 28106 DNA Clean and Concentrator 5-G Zymo Research D4013 Infusion Takara 638948 Zymoclean Gel DNA Recovery Kit Zymo Research D4001 Super Signal West Dura Genesee Scientific 20–301 Super Signal West Femto Genesee Scientific 20–302 Amersham Hybond P 0.2μm PVDF Cytiva 10600021 Bolt 4%–12% Bis-Tris Plus gels Invitrogen NW04120 Nucleofection Buffer MaxCyte EPB-1 Electroporation cuvette MaxCyte SOC-25x3 Experimental models: Cell lines
Protease Inhibitor:Article Title: Local heterochromatin enrichment promotes telomere clustering and PML nuclear body assembly at telomeres.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine RNAiMAX Thermo Fisher Scientific Cat# 13778030 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000015 HMW Poly(I:C) InvivoGen Cat# tlrl-pic Protease QIAGEN Cat# 19157 Benzonase Nuclease EMD Millipore Cat# 70746-3 Formamide Millipore Sigma Cat# S4117 Blocking reagent Roche Cat# 11096176001 Phenol/Chloroform/Isoamyl alcohol (25:24:1) Acros Organics Cat# 32711500 Chloroform Fisher Chemical Cat# C574-1 100% Ethanol Decon Laboratories, Inc. Cat# 2701 Sodium Acetate VWR Cat# 0602-500G HinfI restriction enzyme New England Biolabs Cat# R0155L RsaI restriction enzyme New England Biolabs Cat# R0167 Φ29 Reaction Buffer New England Biolabs Cat# B0269S dNTP mix Qiagen Cat# 201913 Φ29 DNA Polymerase New England Biolabs Cat# M0629L CDP-Star Roche Cat# 11759051001 Pepsin Fisher Scientific Cat# A104-500 Paraformaldehyde, 16% w/v aq soln., methanol free Thermo Scientific Cat. No: 043368.9 RNase A Thermo Scientific Ref# EN0531 Protein A/G Magnetic Beads Thermo Scientific Cat# 88802 Protease inhibitor cocktail Roche Cat# 05892791001 ChIP protease inhibitor mix Diagenode Cat# C12010011 Nucleofection buffer MaxCyte Cat# EPB-1 Cas9 protein IDT Cat# 1081060 Neomycin Thermo Scientific J62671.03 Critical commercial assays BCA Protein Assay ThermoFischer 23227 DIG Oligonucleotide 3 ′ End Labeling Kit, 2ND generation Roche Cat# 03353575910 Qubit High Sensitivity dsDNA Kit Invitrogen Q32851 Click-iT EdU Cell Proliferation Kit for Imaging Thermo Fisher Scientific Cat# C10340 Qiagen DNA Purification Kit Qiagen Cat# 28106 DNA Clean and Concentrator 5-G Zymo Research D4013 Infusion Takara 638948 Zymoclean Gel DNA Recovery Kit Zymo Research D4001 Super Signal West Dura Genesee Scientific 20–301 Super Signal West Femto Genesee Scientific 20–302 Amersham Hybond P 0.2μm PVDF Cytiva 10600021 Bolt 4%–12% Bis-Tris Plus gels Invitrogen NW04120 Nucleofection Buffer MaxCyte EPB-1 Electroporation cuvette MaxCyte SOC-25x3 Experimental models: Cell lines
Chromatin Immunoprecipitation:Article Title: Local heterochromatin enrichment promotes telomere clustering and PML nuclear body assembly at telomeres.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine RNAiMAX Thermo Fisher Scientific Cat# 13778030 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000015 HMW Poly(I:C) InvivoGen Cat# tlrl-pic Protease QIAGEN Cat# 19157 Benzonase Nuclease EMD Millipore Cat# 70746-3 Formamide Millipore Sigma Cat# S4117 Blocking reagent Roche Cat# 11096176001 Phenol/Chloroform/Isoamyl alcohol (25:24:1) Acros Organics Cat# 32711500 Chloroform Fisher Chemical Cat# C574-1 100% Ethanol Decon Laboratories, Inc. Cat# 2701 Sodium Acetate VWR Cat# 0602-500G HinfI restriction enzyme New England Biolabs Cat# R0155L RsaI restriction enzyme New England Biolabs Cat# R0167 Φ29 Reaction Buffer New England Biolabs Cat# B0269S dNTP mix Qiagen Cat# 201913 Φ29 DNA Polymerase New England Biolabs Cat# M0629L CDP-Star Roche Cat# 11759051001 Pepsin Fisher Scientific Cat# A104-500 Paraformaldehyde, 16% w/v aq soln., methanol free Thermo Scientific Cat. No: 043368.9 RNase A Thermo Scientific Ref# EN0531 Protein A/G Magnetic Beads Thermo Scientific Cat# 88802 Protease inhibitor cocktail Roche Cat# 05892791001 ChIP protease inhibitor mix Diagenode Cat# C12010011 Nucleofection buffer MaxCyte Cat# EPB-1 Cas9 protein IDT Cat# 1081060 Neomycin Thermo Scientific J62671.03 Critical commercial assays BCA Protein Assay ThermoFischer 23227 DIG Oligonucleotide 3 ′ End Labeling Kit, 2ND generation Roche Cat# 03353575910 Qubit High Sensitivity dsDNA Kit Invitrogen Q32851 Click-iT EdU Cell Proliferation Kit for Imaging Thermo Fisher Scientific Cat# C10340 Qiagen DNA Purification Kit Qiagen Cat# 28106 DNA Clean and Concentrator 5-G Zymo Research D4013 Infusion Takara 638948 Zymoclean Gel DNA Recovery Kit Zymo Research D4001 Super Signal West Dura Genesee Scientific 20–301 Super Signal West Femto Genesee Scientific 20–302 Amersham Hybond P 0.2μm PVDF Cytiva 10600021 Bolt 4%–12% Bis-Tris Plus gels Invitrogen NW04120 Nucleofection Buffer MaxCyte EPB-1 Electroporation cuvette MaxCyte SOC-25x3 Experimental models: Cell lines
Bicinchoninic Acid Protein Assay:Article Title: Local heterochromatin enrichment promotes telomere clustering and PML nuclear body assembly at telomeres.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine RNAiMAX Thermo Fisher Scientific Cat# 13778030 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000015 HMW Poly(I:C) InvivoGen Cat# tlrl-pic Protease QIAGEN Cat# 19157 Benzonase Nuclease EMD Millipore Cat# 70746-3 Formamide Millipore Sigma Cat# S4117 Blocking reagent Roche Cat# 11096176001 Phenol/Chloroform/Isoamyl alcohol (25:24:1) Acros Organics Cat# 32711500 Chloroform Fisher Chemical Cat# C574-1 100% Ethanol Decon Laboratories, Inc. Cat# 2701 Sodium Acetate VWR Cat# 0602-500G HinfI restriction enzyme New England Biolabs Cat# R0155L RsaI restriction enzyme New England Biolabs Cat# R0167 Φ29 Reaction Buffer New England Biolabs Cat# B0269S dNTP mix Qiagen Cat# 201913 Φ29 DNA Polymerase New England Biolabs Cat# M0629L CDP-Star Roche Cat# 11759051001 Pepsin Fisher Scientific Cat# A104-500 Paraformaldehyde, 16% w/v aq soln., methanol free Thermo Scientific Cat. No: 043368.9 RNase A Thermo Scientific Ref# EN0531 Protein A/G Magnetic Beads Thermo Scientific Cat# 88802 Protease inhibitor cocktail Roche Cat# 05892791001 ChIP protease inhibitor mix Diagenode Cat# C12010011 Nucleofection buffer MaxCyte Cat# EPB-1 Cas9 protein IDT Cat# 1081060 Neomycin Thermo Scientific J62671.03 Critical commercial assays BCA Protein Assay ThermoFischer 23227 DIG Oligonucleotide 3 ′ End Labeling Kit, 2ND generation Roche Cat# 03353575910 Qubit High Sensitivity dsDNA Kit Invitrogen Q32851 Click-iT EdU Cell Proliferation Kit for Imaging Thermo Fisher Scientific Cat# C10340 Qiagen DNA Purification Kit Qiagen Cat# 28106 DNA Clean and Concentrator 5-G Zymo Research D4013 Infusion Takara 638948 Zymoclean Gel DNA Recovery Kit Zymo Research D4001 Super Signal West Dura Genesee Scientific 20–301 Super Signal West Femto Genesee Scientific 20–302 Amersham Hybond P 0.2μm PVDF Cytiva 10600021 Bolt 4%–12% Bis-Tris Plus gels Invitrogen NW04120 Nucleofection Buffer MaxCyte EPB-1 Electroporation cuvette MaxCyte SOC-25x3 Experimental models: Cell lines
End Labeling:Article Title: Local heterochromatin enrichment promotes telomere clustering and PML nuclear body assembly at telomeres.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine RNAiMAX Thermo Fisher Scientific Cat# 13778030 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000015 HMW Poly(I:C) InvivoGen Cat# tlrl-pic Protease QIAGEN Cat# 19157 Benzonase Nuclease EMD Millipore Cat# 70746-3 Formamide Millipore Sigma Cat# S4117 Blocking reagent Roche Cat# 11096176001 Phenol/Chloroform/Isoamyl alcohol (25:24:1) Acros Organics Cat# 32711500 Chloroform Fisher Chemical Cat# C574-1 100% Ethanol Decon Laboratories, Inc. Cat# 2701 Sodium Acetate VWR Cat# 0602-500G HinfI restriction enzyme New England Biolabs Cat# R0155L RsaI restriction enzyme New England Biolabs Cat# R0167 Φ29 Reaction Buffer New England Biolabs Cat# B0269S dNTP mix Qiagen Cat# 201913 Φ29 DNA Polymerase New England Biolabs Cat# M0629L CDP-Star Roche Cat# 11759051001 Pepsin Fisher Scientific Cat# A104-500 Paraformaldehyde, 16% w/v aq soln., methanol free Thermo Scientific Cat. No: 043368.9 RNase A Thermo Scientific Ref# EN0531 Protein A/G Magnetic Beads Thermo Scientific Cat# 88802 Protease inhibitor cocktail Roche Cat# 05892791001 ChIP protease inhibitor mix Diagenode Cat# C12010011 Nucleofection buffer MaxCyte Cat# EPB-1 Cas9 protein IDT Cat# 1081060 Neomycin Thermo Scientific J62671.03 Critical commercial assays BCA Protein Assay ThermoFischer 23227 DIG Oligonucleotide 3 ′ End Labeling Kit, 2ND generation Roche Cat# 03353575910 Qubit High Sensitivity dsDNA Kit Invitrogen Q32851 Click-iT EdU Cell Proliferation Kit for Imaging Thermo Fisher Scientific Cat# C10340 Qiagen DNA Purification Kit Qiagen Cat# 28106 DNA Clean and Concentrator 5-G Zymo Research D4013 Infusion Takara 638948 Zymoclean Gel DNA Recovery Kit Zymo Research D4001 Super Signal West Dura Genesee Scientific 20–301 Super Signal West Femto Genesee Scientific 20–302 Amersham Hybond P 0.2μm PVDF Cytiva 10600021 Bolt 4%–12% Bis-Tris Plus gels Invitrogen NW04120 Nucleofection Buffer MaxCyte EPB-1 Electroporation cuvette MaxCyte SOC-25x3 Experimental models: Cell lines
Imaging:Article Title: Local heterochromatin enrichment promotes telomere clustering and PML nuclear body assembly at telomeres.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine RNAiMAX Thermo Fisher Scientific Cat# 13778030 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000015 HMW Poly(I:C) InvivoGen Cat# tlrl-pic Protease QIAGEN Cat# 19157 Benzonase Nuclease EMD Millipore Cat# 70746-3 Formamide Millipore Sigma Cat# S4117 Blocking reagent Roche Cat# 11096176001 Phenol/Chloroform/Isoamyl alcohol (25:24:1) Acros Organics Cat# 32711500 Chloroform Fisher Chemical Cat# C574-1 100% Ethanol Decon Laboratories, Inc. Cat# 2701 Sodium Acetate VWR Cat# 0602-500G HinfI restriction enzyme New England Biolabs Cat# R0155L RsaI restriction enzyme New England Biolabs Cat# R0167 Φ29 Reaction Buffer New England Biolabs Cat# B0269S dNTP mix Qiagen Cat# 201913 Φ29 DNA Polymerase New England Biolabs Cat# M0629L CDP-Star Roche Cat# 11759051001 Pepsin Fisher Scientific Cat# A104-500 Paraformaldehyde, 16% w/v aq soln., methanol free Thermo Scientific Cat. No: 043368.9 RNase A Thermo Scientific Ref# EN0531 Protein A/G Magnetic Beads Thermo Scientific Cat# 88802 Protease inhibitor cocktail Roche Cat# 05892791001 ChIP protease inhibitor mix Diagenode Cat# C12010011 Nucleofection buffer MaxCyte Cat# EPB-1 Cas9 protein IDT Cat# 1081060 Neomycin Thermo Scientific J62671.03 Critical commercial assays BCA Protein Assay ThermoFischer 23227 DIG Oligonucleotide 3 ′ End Labeling Kit, 2ND generation Roche Cat# 03353575910 Qubit High Sensitivity dsDNA Kit Invitrogen Q32851 Click-iT EdU Cell Proliferation Kit for Imaging Thermo Fisher Scientific Cat# C10340 Qiagen DNA Purification Kit Qiagen Cat# 28106 DNA Clean and Concentrator 5-G Zymo Research D4013 Infusion Takara 638948 Zymoclean Gel DNA Recovery Kit Zymo Research D4001 Super Signal West Dura Genesee Scientific 20–301 Super Signal West Femto Genesee Scientific 20–302 Amersham Hybond P 0.2μm PVDF Cytiva 10600021 Bolt 4%–12% Bis-Tris Plus gels Invitrogen NW04120 Nucleofection Buffer MaxCyte EPB-1 Electroporation cuvette MaxCyte SOC-25x3 Experimental models: Cell lines
DNA Purification:Article Title: Local heterochromatin enrichment promotes telomere clustering and PML nuclear body assembly at telomeres.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine RNAiMAX Thermo Fisher Scientific Cat# 13778030 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000015 HMW Poly(I:C) InvivoGen Cat# tlrl-pic Protease QIAGEN Cat# 19157 Benzonase Nuclease EMD Millipore Cat# 70746-3 Formamide Millipore Sigma Cat# S4117 Blocking reagent Roche Cat# 11096176001 Phenol/Chloroform/Isoamyl alcohol (25:24:1) Acros Organics Cat# 32711500 Chloroform Fisher Chemical Cat# C574-1 100% Ethanol Decon Laboratories, Inc. Cat# 2701 Sodium Acetate VWR Cat# 0602-500G HinfI restriction enzyme New England Biolabs Cat# R0155L RsaI restriction enzyme New England Biolabs Cat# R0167 Φ29 Reaction Buffer New England Biolabs Cat# B0269S dNTP mix Qiagen Cat# 201913 Φ29 DNA Polymerase New England Biolabs Cat# M0629L CDP-Star Roche Cat# 11759051001 Pepsin Fisher Scientific Cat# A104-500 Paraformaldehyde, 16% w/v aq soln., methanol free Thermo Scientific Cat. No: 043368.9 RNase A Thermo Scientific Ref# EN0531 Protein A/G Magnetic Beads Thermo Scientific Cat# 88802 Protease inhibitor cocktail Roche Cat# 05892791001 ChIP protease inhibitor mix Diagenode Cat# C12010011 Nucleofection buffer MaxCyte Cat# EPB-1 Cas9 protein IDT Cat# 1081060 Neomycin Thermo Scientific J62671.03 Critical commercial assays BCA Protein Assay ThermoFischer 23227 DIG Oligonucleotide 3 ′ End Labeling Kit, 2ND generation Roche Cat# 03353575910 Qubit High Sensitivity dsDNA Kit Invitrogen Q32851 Click-iT EdU Cell Proliferation Kit for Imaging Thermo Fisher Scientific Cat# C10340 Qiagen DNA Purification Kit Qiagen Cat# 28106 DNA Clean and Concentrator 5-G Zymo Research D4013 Infusion Takara 638948 Zymoclean Gel DNA Recovery Kit Zymo Research D4001 Super Signal West Dura Genesee Scientific 20–301 Super Signal West Femto Genesee Scientific 20–302 Amersham Hybond P 0.2μm PVDF Cytiva 10600021 Bolt 4%–12% Bis-Tris Plus gels Invitrogen NW04120 Nucleofection Buffer MaxCyte EPB-1 Electroporation cuvette MaxCyte SOC-25x3 Experimental models: Cell lines
Electroporation:Article Title: Local heterochromatin enrichment promotes telomere clustering and PML nuclear body assembly at telomeres.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine RNAiMAX Thermo Fisher Scientific Cat# 13778030 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000015 HMW Poly(I:C) InvivoGen Cat# tlrl-pic Protease QIAGEN Cat# 19157 Benzonase Nuclease EMD Millipore Cat# 70746-3 Formamide Millipore Sigma Cat# S4117 Blocking reagent Roche Cat# 11096176001 Phenol/Chloroform/Isoamyl alcohol (25:24:1) Acros Organics Cat# 32711500 Chloroform Fisher Chemical Cat# C574-1 100% Ethanol Decon Laboratories, Inc. Cat# 2701 Sodium Acetate VWR Cat# 0602-500G HinfI restriction enzyme New England Biolabs Cat# R0155L RsaI restriction enzyme New England Biolabs Cat# R0167 Φ29 Reaction Buffer New England Biolabs Cat# B0269S dNTP mix Qiagen Cat# 201913 Φ29 DNA Polymerase New England Biolabs Cat# M0629L CDP-Star Roche Cat# 11759051001 Pepsin Fisher Scientific Cat# A104-500 Paraformaldehyde, 16% w/v aq soln., methanol free Thermo Scientific Cat. No: 043368.9 RNase A Thermo Scientific Ref# EN0531 Protein A/G Magnetic Beads Thermo Scientific Cat# 88802 Protease inhibitor cocktail Roche Cat# 05892791001 ChIP protease inhibitor mix Diagenode Cat# C12010011 Nucleofection buffer MaxCyte Cat# EPB-1 Cas9 protein IDT Cat# 1081060 Neomycin Thermo Scientific J62671.03 Critical commercial assays BCA Protein Assay ThermoFischer 23227 DIG Oligonucleotide 3 ′ End Labeling Kit, 2ND generation Roche Cat# 03353575910 Qubit High Sensitivity dsDNA Kit Invitrogen Q32851 Click-iT EdU Cell Proliferation Kit for Imaging Thermo Fisher Scientific Cat# C10340 Qiagen DNA Purification Kit Qiagen Cat# 28106 DNA Clean and Concentrator 5-G Zymo Research D4013 Infusion Takara 638948 Zymoclean Gel DNA Recovery Kit Zymo Research D4001 Super Signal West Dura Genesee Scientific 20–301 Super Signal West Femto Genesee Scientific 20–302 Amersham Hybond P 0.2μm PVDF Cytiva 10600021 Bolt 4%–12% Bis-Tris Plus gels Invitrogen NW04120 Nucleofection Buffer MaxCyte EPB-1 Electroporation cuvette MaxCyte SOC-25x3 Experimental models: Cell lines
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